Figure 5. Ectopic insertion of TAS fragment is sufficient to cause nucleosome instability.

1. ChIP‐qPCR analysis of H3 at endogenous TAS and a fragment spanning the TAS region from 115 to 905 bp (relative to telomeric repeat) inserted into the leu1 ⁺ locus (see scheme). The TAS fragment was inserted into a strain that lacks endogenous TAS (see text) 12. Shown are ChIP analyses for WT (left) and ccq1∆ (right; note different the scale of the y‐axis) (n = 9–10 independent experiments except for ectopic TAS in ccq1∆ strain where n = 3).
2. ChIP‐qPCR analysis of H3 at reporter genes (ura4 ⁺ and his3 ⁺) at various chromosomal locations (see schemes) in WT cells. TAS1, TAS2, and TAS3 correspond to position 116, 2,851, and 6,291 bp (relative to telomeric repeats), respectively (n = 3 independent experiments).

Data information: In (A, B), data are represented as mean ± SEM (normalization as in Fig 2B and C).