Figure 7. Subtelomeric DNA promotes recombination in the absence of Ccq1.

1. Genomic copy number of his3 ⁺, ura4 ⁺, and TAS1 in indicated strains harboring the reporter genes tel1L::his3 ⁺ and tel2L::ura4 ⁺. Cultures from individual WT and freshly generated knockout clones (ccq1Δ, n = 16; clr4Δ, n = 5; ccq1Δ clr4Δ, n = 5) were pre‐grown on selective media for several days and inoculated at day 0 to grow in liquid media with regular back‐dilution (every 24 h, approximately 7 generations). Samples were taken at indicated harvest times, and relative copy numbers of genomic regions were assessed by qPCR (normalization against intrachromosomal loci). Black and rainbow color lines indicate WT strains and individual ccq1 ⁺ deletion mutants (clones), respectively.
2. qPCR analysis as in (A) but with strains harboring the minichromosome Ch16 m23::ura4 ⁺ (ccq1Δ, n = 14). Since Ch16 is unstable in ccq1Δ cells (see Appendix Fig S3A and B and 37, m23::ura4 ⁺ has been normalized to minichromosome levels (details on normalization, see Materials and Methods). Note that Ch16 does not harbor TAS sequences.