Skip to main content
. Author manuscript; available in PMC: 2019 Jan 7.
Published in final edited form as: J Med Chem. 2018 Mar 23;61(7):3193–3208. doi: 10.1021/acs.jmedchem.8b00261

Figure 6.

Figure 6.

(R)-Enantiomer N54 is more active than (S)-enantiomer N55 in cellular assays. (A, B) Western blot analyses of (A) SKBR3 and BT474 cells, (B) MCF7 and MDA-MB231 cells treated with the indicated concentration of N54, N55, KDM5-C70, or DMSO control for 3 days. Representative data from biological duplicate experiments performed in duplicate are shown. (C, D) Colony-formation assays of MCF-7 cells treated with DMSO, KDM5-C70 (1 μM), and N54 and N55 (1 or 10 μM). Shown are (C) representative images and (D) quantification of biological triplicate experiments. “Intensity %” indicates the measured intensity of cell confluency using ImageJ. Error bars denote SEM: (***) p < 0.001.