Skip to main content
. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Nat Protoc. 2018 Nov;13(11):2535–2556. doi: 10.1038/s41596-018-0048-z

Figure 4. DNA cleavage frequency profiles extracted from HRF experiments.

Figure 4.

According to our HRF data quantification methodology the lane profile curve in Figure 3B was fitted by a combination of Gaussian functions. The areas of these individual Gaussian functions represent the individual band intensities and hence DNA cleavage frequency values. These quantified DNA cleavage frequency values are plotted here for both unconstrained and constrained fitting results presented in Figure 3B. Correspondence between the DNA sequence and HRF profile was identified using Maxam-Gilbert sequencing reactions’ products run alongside the HRF products on the gel. Irregularities in the values resulting from the unconstrained fitting are visible. Zero on the x-axis marks the nucleotide positioned on the central dyad symmetry axis of nucleosome.