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. Author manuscript; available in PMC: 2019 Jan 7.
Published in final edited form as: Mol Cell. 2017 Sep 21;68(1):89–103.e7. doi: 10.1016/j.molcel.2017.08.025

Figure 5. Naa10p and Dnmt1 Co-occupy ICRs/DMRs.

Figure 5.

(A) Naa10p co-localizes with Dnmt1 at multiple ICRs/DMRs in WT mouse ESCs. ChIP with the Dnmt1 Ab from WT#1 and KO#1 ESC clones was carried out again with Naa10p Ab, followed by qPCR with primers against the ICRs/DMRs indicated. Biological repeats using independent ESC clones WT#2 and KO#2 are shown in Figure S5.

(B) Naa10p co-localizes with Dnmt1 at multiple ICRs/DMRs in a WT mouse embryo. ChIP with the Dnmt1 Ab in Figure 4D from embryos of WT (153) and Naa10-KO (155) was carried out again with Naa10p Ab, followed by qPCR with primers against the ICRs/DMRs indicated.

(A and B) Error bars represent SD determined from triplicate PCR reactions. Asterisks indicate a statistical difference calculated by two-tailed t test: *p < 0.05, **p < 0.01.

See also Figure S5.