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. 2018 Feb 7;149(3):253–261. doi: 10.1093/ajcp/aqx162

Table 3.

Summarized Key Studies Showing PD-L1 Expression in Breast Carcinoma Cells Assessed Using Immunohistochemistry Staining and Its Clinicopathologic Relevance

First Author (Year) No. of Cases Antibody Clone Cutoff for PD-L1 Positivity (Cell Compartment Evaluated) PD-L1–Positive Rate Clinicopathologic Variables Significantly Associated With Positive PD-L1 Expression Survival Variables Associated With PD-L1 Positivity
Ghebeh (2006)27 44 breast cancer samples (frozen section) MIH1 Any positive cells (cell membrane and/or cytoplasm) 34.0% ER–, PR– NA
Mittendorf (2014)28 120 TNBCs (TMA) 5H1 >5% (cell membrane) 19.0% NA NA
Muenst (2014)14 650 breast cancer samples (TMA) Rabbit polyclonal antibody H-score ≥100 (cell membrane and/or cytoplasm) 23.4% Higher grade and Ki-67 index, larger size, positive nodal status, ER– Shorter OS in univariate and multivariate analysis
Qin (2015)16 870 breast cancer samples (TMA) Rabbit polyclonal antibody >5% (cell membrane and/or cytoplasm) 21.7% (55.9% in TNBC) Larger size, higher grade, LVI, ER–, PR– Shorter DFS, OS
Ali (2015)15 3,916 breast cancer samples (TMA) Rabbit polyclonal antibody >1% (not specified) 1.7% Basal-like subtype Better DFS (P = .08) in ER– tumors
Guo (2016)23 183 TNBCs (TMA) SP142 Not specified (cell membrane and/or cytoplasm) 8.7% Higher grade Not associated with survival
Cimino-Mathews (2016)21 45 breast cancer samples (whole sections) 5H1 ≥5% (cell membrane) 21.0% Higher grade Less likely to have distant metastasis
Beckers (2016)20 161 TNBCs (TMA) E1L3N ≥1% and ≥5% (cell membrane) 64.0% and 60.0%, respectively Not associated with any variables Not associated with survival
≥1% and ≥5% (cell cytoplasm) 80.0% and 77.8%, respectively Not associated with any variables Lower risk of cancer- specific death
Bae (2016)18 465 breast cancer samples (TMA) E1L3N H-score ≥100 (cell membrane and/or cytoplasm) 13.5% Higher grade and Ki-67 index, negative nodal status, ER–, PR–, HER2+ Longer DFS and OS in univariate analysis
Li (2016)24 136 TNBCs (whole sections) E1L3N Any positive cells (cell membrane) 21.0% NA Not associated with DFS
Baptista (2016)19 192 breast cancer samples (TMA) Rabbit polyclonal antibody Allred score >0 (cell membrane and/or cytoplasm) 56.6% Larger size, positive nodal status, ER–, distant recurrence Better OS
Li (2016)25 501 breast cancer samples (whole sections) ab58810 H-score ≥100 (cell membrane and/or cytoplasm) 46.1% Higher grade, aggressive intrinsic subtype, ER–, PR– Shorter OS
Mori (2017)43 248 TNBCs (whole sections) E1L3N ≥1% (cell membrane) 41.5% Higher grade, and Ki-67 index Not associated with prognosis

DFS, disease-free survival; ER, estrogen receptor; HER2, human epidermal growth factor receptor 2; LVI, lymphovascular invasion; NA, not available; OS, overall survival; PD-L1, programmed death ligand 1; PR, progesterone receptor; TMA, tissue microarray; TNBC, triple-negative breast cancer.