Fig. 7.

BTB permeability. Biotin was injected underneath the testicular capsules of control (Ctrl) and immunized (Imm.) WT mice. After 30 min, paraffin sections were stained with FITC-conjugated streptavidin (left panels), after which the sections were counterstained with DAPI (middle panels). Images of biotin and DAPI were merged (right panels). Arrows, arrowheads, and asterisks indicate basal compartment, interstitial spaces, and adluminal compartments of seminiferous tubules, respectively. Images represent at least three experiments on three mice. Bar = 20 μm.