FIG 3.

MeltPro Myco assay for mixed-infection detection. (A) Two coexisting species, M. tuberculosis (MTB) and M. chelonae (MCH), were detected in one channel (FAM). (B) Two coexisting species, MTB and M. intracellulare (MIN), were detected in two channels (FAM and Cy5). The overall template concentration was 5 × 10³ copies per reaction. Melting curves of artificial plasmid templates containing two species with various ratios (0:100, 1:99, 5:95, 25:75, 50:50, 75:25, 95:5, 99:1 to 100:0) are shown (gray lines) together with melting curves of the mixed template containing 1% MTB (red line), 1% of MCH (A), or 1% of MIN (B) (blue line), and NTC (dashed line).