TABLE 2.
Validation of 18 mixed infections found in 1,163 clinical isolates
| Sample | Mixed infection bacteria | Discrepant bpa | Validation assayb |
|---|---|---|---|
| GZ k1624623 | M. avium-M. abscessus | 38 (AGE) | ITS |
| GZ k1624078 | M. intracellulare-M. abscessus | 38 (AGE) | ITS |
| GZ k1624914 | M. tuberculosis-M. abscessus | 36 (AGE) | ITS |
| GZ k1621849 | M. tuberculosis-M. abscessus | 36 (AGE) | ITS |
| GZ k1623927 | M. tuberculosis-M. abscessus | 36 (AGE) | ITS |
| GZ k1620589 | M. tuberculosis-M. abscessus | 36 (AGE) | ITS |
| GZ k1621157 | M. tuberculosis-M. abscessus | 36 (AGE) | ITS |
| GZ k1617424 | M. xenopi-M. lentiflavum | 25 (PAGE) | ITS |
| GZ k1623332 | M. fortuitum-M. senegalense | 14 (PAGE) | ITS |
| GZ k1618570 | M. tuberculosis-M. gordonae | 10 (PAGE) | ITS |
| GZ k1621958 | M. tuberculosis-M. avium | 2 | IS6110, IS901 |
| GZ k1616819 | M. tuberculosis-M. intracellulare | 2 | IS6110, DT1 |
| XM TA15018 | M. tuberculosis-M. intracellulare | 2 | IS6110, DT1 |
| XJ 5504 | M. tuberculosis-M. intracellulare | 2 | IS6110, DT1 |
| XJ 5541 | M. tuberculosis-M. intracellulare | 2 | IS6110, DT1 |
| XJ 5551 | M. tuberculosis-M. intracellulare | 2 | IS6110, DT1 |
| XJ 5614 | M. tuberculosis-M. intracellulare | 2 | IS6110, DT1 |
| XJ 5563 | M. tuberculosis-M. intracellulare | 2 | IS6110, DT1 |
a
Discrepant base pairs between two amplicon fragments amplified from mixed strains using panmycobacterial ITS primers, as previously described (17). Agarose gel electrophoresis (AGE) or polyacrylamide gel electrophoresis (PAGE) was used to separate two different amplicons based on corresponding discrepant base pairs.
b
For mixed infections with two different amplicons that can be separated by AGE or PAGE, both PCR products were subjected to ITS sequencing. For M. tuberculosis-M. avium and M. tuberculosis-M. intracellulare, M. tuberculosis, M. avium, and M. intracellulare were confirmed by using their respective specific genes, i.e., IS6110, IS901, and DT1.