FIG 2.

Characteristics and outcomes of the clinical field studies. (A) Distribution of HRP2 and pan LDH concentrations with respect to parasite density (0.01 to 10⁶ parasites/µl). The results show a box plot with the median (horizontal line), the 25th and 75th percentiles (boxes), the 10th and 90th percentiles (error bars), and the outliers (open circles). The dotted lines indicate cutoff values, as follows: 2.3 pg/ml for HRP2, 47.8 pg/ml for pan LDH, and 75.1 pg/ml for P. vivax LDH. The face concentration values were used in this analysis for specimens with concentration at more than ULOQ. (B) Distribution of HRP2, pan LDH, and P. vivax LDH amount per a circulating parasite respect to Plasmodium infection. Limited analysis was performed on specimens with quantifiable malaria antigens, because the accurate quantification of HRP2 is not possible at more than approximately 100 parasites/µl (P. falciparum-positive for HRP2, n = 39; P. falciparum-positive for pan LDH, n = 30; P. vivax-positive for P. vivax LDH, n = 41). The results show the median (horizontal line), the 25th and 75th percentiles (boxes), the 10th and 90th percentiles (error bars), and the outliers (open circles). The two-tailed Mann–Whitney U-test was used to compare the distributions of two unmatched groups.