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. Author manuscript; available in PMC: 2019 Jan 7.
Published in final edited form as: Biochemistry. 2017 Nov 28;56(49):6423–6433. doi: 10.1021/acs.biochem.7b00700

Figure 1.

Figure 1.

Comparison of wild-type (wt) TIMP-2 (pT2MO1 coding sequence) and codon-optimized (CO) rhTIMP-2-6XHis (COT2his) cDNA and protein sequences. (A) ClustalW (MacVector version 15.5.0) pairwise nucleotide alignment and comparison of the wt TIMP-2 (pT2MO1) and codon-optimized rhTIMP-2-6XHis (COT2his) cDNA sequences using a 10-nucleotide window indicating changes made to and from G and C nucleotides. (B) Comparison of the % GC content in the pT2MO1 (blue) and COT2his (red) lines in 10-nucleotide sequence windows using the MacVector software. (C) Alignment of the translated protein sequences from wt (pT2MO1) and codon-optmized rhTIMP-2-6XHis (COT2his) cDNA sequences.