Skip to main content
. Author manuscript; available in PMC: 2019 Jan 7.
Published in final edited form as: Biochemistry. 2017 Nov 28;56(49):6423–6433. doi: 10.1021/acs.biochem.7b00700

Table 1.

Effects of cDNA Construct (codon optimization), Transfection, Cell Line, and Culture Conditions on rhTIMP-2-EK-6XHis Expressiona

sample construct cell transfection culture conditions yield (mg/L) standard deviation
1 TIMP-2-EK-6XHis HEK-293-F transient suspension 3 0.3
2 TIMP-2-EK-6XHis HEK-293 stable monolayer 3 0.3
3 TIMP-2-EK-6XHis CHO-S transient suspension 5.5 0.6
4 TIMP-2-EK-6XHis CHO-S stable suspension 8 0.8
5 coTIMP-2-6XHis HEK-293-F stable suspension/shaker 15 1.1
6 coTIMP-2-6XHis HEK-293-F stable suspension/shaker 20.8 1.2
7 coTIMP-2-6XHis HEK-293-F stable suspension/spinner 40 1.6
8 coTIMP-2-6XHis HEK-293-F stable suspension/wave 35 1.7
a

Production yields are significantly higher in stably transfected HEK-293-F cells vs transient transfections and using codon-optimized TIMP-2. Yields are reported for all combinations of cDNA, cell lines, and culture conditions (n = 3).