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. Author manuscript; available in PMC: 2019 Jan 7.
Published in final edited form as: Life Sci. 2018 Apr 13;203:39–47. doi: 10.1016/j.lfs.2018.04.017

Fig. 5.

Fig. 5.

Induction of the fibrogenic factors expression in HSCs by recombinant Slit2 (rSlit2) via PI3K/Akt pathway. (A) Representative Western blot profile of phosphorylated or total PI3K and Akt in JS1 cells with rSlit2 (100 ng/mL) treatment for 0 h, 30 min, 1 h and 2 h are shown. Results are expressed as ratios of semiquantitative analyzed phosphorylated PI3K or Akt signals to their corresponding total proteins. rSlit2 treatment increases PI3K and Akt phosphorylation in JS1 cells. Data are presented as mean ± SD of N = 3 per group in three independent experiments. **P < 0.01, and ***P < 0.001 compared to 0 h; (B) Representative Western blot profile for the effect of rSlit2 (100 ng/mL) on the protein expression of Col-1, TGF-β1 and CTGF in JS1 cells with or without LY294002 (25 μM) pretreatment. Results are expressed as ratios of Col-1, TGF-β1, CTGF and p-AKT signals to that of GAPDH. Data are presented as mean ± SD of N = 3 per group in three independent experiments. ***P < 0.001 compared to NC; ### P < 0.001 compared to cells treated with rSlit2; NC: control cells treated with vehicle; rSlit2: cells treated with rSlit2 (100 ng/mL); rSlit2 + LY294002: cells treated with LY294002 (25 μM) and rSlit2 (100 ng/mL).