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. 2018 Oct 30;70(2):519–528. doi: 10.1093/jxb/ery374

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — CARK1 phosphorylates RCAR12, RCAR13, and RCAR14. (A) Alignment of the phosphorylation sites with subfamily III receptor RCARs (R11–R14). (B–D) In vitro kinase assay. Phosphorylation of GST–RCAR12/13/14 (abbreviated as R12/13/14) by CARK1-KD (B). Phosphorylation of His-RCAR12/13/14 by CARK1-KD (C). Determination of the potential phosphorylation site of RCAR12/13/14 (D). Anti-phosphothreoinine antibody was used for the analysis of the western blot. The loading amounts of proteins were visualized on the Coomassie Brilliant Blue- (CBB) stained gel (bottom panel). Experiments were repeated at least three times.