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. 2018 Oct 30;70(2):519–528. doi: 10.1093/jxb/ery374

Fig. 4.

Fig. 4.

RCARs act downstream of CARK1 in the ABA-induced inhibition of seed germination and root length. (A) Seed germination assay. Seeds (>100) were scored 3 d after stratification on MS medium supplemented with different concentrations of ABA. Values are the means ±SD (n=3). (B) The root architecture of seedlings was documented at 7 d post-transfer to media (1/2 MS, 1% sucrose) with or without ABA. Seedlings were 3 d old at the time of transfer and had equal root lengths at that time. (C) Statistical analysis of ABA-inhibited root growth in (B). Data are the means ±SD (n=20). Col-0, Columbia ecotype; cark1, CARK1 T-DNA mutant; CARK1-OE 19#, plants overexpressing CARK1 in Col-0; Ler (Landsberg erecta ecotype); CARK1:1124, overexpression of CARK1 in the 1124 quadruple mutant plants; 1124, ABA receptor pyr1pyl1pyl2pyl4 quadruple mutant. *P<0.05, **P<0.01, Student’s t-test. All physiological analyses were conducted in triplicate.