Fig. 6.
CARK1 and subfamily III ABA receptors synergistically regulate the expression of the reporter gene driven by the RAB18 or RAB29B promoter. Protoplasts of cark1 were transfected with 10 μg of effector DNA CARK1 (A and B) or CARK1N204A (abbreviated as CARK1m; C and D) for expression of the different RCARs (RCAR12/13/14) in the absence and presence of 10 μM exogenous ABA. As a negative control, empty effector vectors were used, taking the place of the effector plasmid. The relative activity of the vector control was set at 1. Experiments were repeated at least three times. Vertical bars indicate ±SD of three replicates.