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. 2018 Nov 24;70(2):435–446. doi: 10.1093/jxb/ery385

Fig. 7.

Fig. 7.

ABF1, ABF2, ABF3, ABF4, and DPBF4 can all activate the expression of ADF5 via the ABRE core motif when transiently expressed in tobacco leaves. Leaves of N. benthamiana were co-infiltrated with ADF5p311-Luc, the 311 bp region downstream of the ADF5 promoter tagged with firefly luciferase. ADF5p311m indicates the change from ACGA/G to AAGA/G at position –203 to –206 bp. Similarly, ADF5p311ms1 indicates the change from ACGC/T to TAAG/T at positions –5 to –78 bp, –147 to –150 bp, +44 to +47 bp, and +94 to +97 bp; and ADF5p311ms2 indicates the change from ACGC/T to TAAG/T at positions –75 to –78 bp, –147 to –150 bp, +44 to +47 bp, and +94 to +97 bp, and the change from ACGA/G to AAGA/G at positions –1 to –4 bp, –135 to –138 bp, and –203 to –206 bp. All mutations in ACG motifs abolished the binding between AREB/ABF transcription factors and the promoter of ADF5.