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. Author manuscript; available in PMC: 2019 Jan 7.
Published in final edited form as: J Immunother. 2010 Oct;33(8):840–847. doi: 10.1097/CJI.0b013e3181f05b91

TABLE 1.

An Example of Coculture Results to Determine Specific Activity

Targets
Interferon-γ (pg/ml) > Indicates off-scale Effector Tissue Culture Lines
A1, A24		 Tissue Culture Lines
A2, A3		 Tissue Culture Autologous-2183 Shared Antigen-2188 Fresh Tumor Autologous-2188 Shared Antigen-2183 Fresh Tumor (A1, A3) Mismatch-2188 Shared Antigen-2183
None 888 938 526 624 2183 2188 2088
None 0 0 0 0 0 0 0 11
MART reactive (+) control 6 6 16 6900 > 31,870 8940 26 53
Gp100 reactive (+) control 0 0 0 2880 2570 6490 1 0
(−) control 0 0 0 0 0 0 0 0
2188 (A2, A11) fragments
 F3 0 9 14 238 69 33 310 55
 F4 0 10 0 0 0 0 111 0
 F5 0 0 0 199 183 231 50 16
 F6 18 37 9 0 5 3 171 33
 F7 37 486 5 33 60 37 294 98
 F8 26 394 28 252 159 522 291 100
2188 (A2, A11) digest
 W1 25 13 18 16 35 9 518 284
 W2 32 12 15 9 8 8 114 31
 W3 0 0 2 70 0 103 372 51
 W4 10 0 0 172 0 0 500 191
2183 (A1, A2) fragments
 F2 11 87 30 > 2301 2740 1470 78 83
 F3 92 326 52 5950 14,820 5040 176 198
 F4 1527 275 222 590 233 565 716 1138
 F5 157 41 50 130 84 1300 126 147
 F7 1734 435 306 653 469 672 > 2776 > 3076
 F8 111 44 32 139 91 194 128 115

Bold values indicate tumor recognition as defined in Methods.