Table 1. PCR primer sequences for gene expression.
Gene Target | Primer orientation | Sequence (5'-3') | Amplicon size (bp) | Reference |
---|---|---|---|---|
IFN-γ | Forward | TGAACATGATGGATCGTTGG | 182 | [63] |
IFN-γ | Reverse | CACTTTGCTGGCAGTGTTGT | This study | |
IL-6 | Forward | CACCTGTTTGGCTTTTAGCA | 187 | This study |
IL-6 | Reverse | GCAGAGCTTGTATGGCTCCT | This study | |
IL-17A | Forward | TCCCTAATGAGGATGCCAAC | 173 | This study |
IL-17A | Reverse | TTGGAAGGAAGTGGAGCAGT | This study | |
GAPDH | Forward | AACTTTGGCATTGTGGAAGG | 188 | This study |
GAPDH | Reverse | GTGAGCTTCCCATTCAGCTC | This study |
Gene targets with corresponding forward and reverse primer and amplicon size (bp).