Table 4. Overall pathogen-specific statistical performance in Monoplex Serology in corresponding reference panels.
| RP | pathogen | antigen | cut -off1 (MFI) | algorithm | specificity (95% CI) |
sensitivity (95% CI) |
kappa (95% CI) |
|---|---|---|---|---|---|---|---|
| Ia | HBV | HBe | 30 | AND | 100.0 (97.4–100.0) | 92.3 (84.8–96.9) | 0.94 (0.89–0.98) |
| HBc | 30 | ||||||
| Ib | HBV | HBe | 90 | AND | 100.0 (96.4–100.0) | 93.0 (83.0–98.1) | 0.94 (0.89–100.0) |
| HBc | 110 | ||||||
| II | HCV | Core | 100 | AND / OR | 96.2 (90.4–98.9) | 98.0 (89.4–100.0) | 0.93 (0.86–0.99) |
| NS3 | 40 | ||||||
| Core | 30 | AND | 100.0 (96.5–100.0) | 84.0 (70.9–92.8) | 0.88 (0.79–0.96) | ||
| NS3 | 30 | ||||||
| III | HTLV-1 | gag | 50 | AND / OR | 100 (96.4–100.0) | 94.0 (87.4–97.8) | 0.94 (0.89–0.99) |
| env | 50 | ||||||
| IV | T. gondii | p22 | 77 | AND / OR | 92.0 (80.8–97.8) | 91.2 (85.5–95.2) | 0.79 (0.69–0.88) |
| sag1 | 100 |
1 cut-offs determined by optimizing specificity and sensitivity
CI: confidence interval
RP: Reference panel
MFI: median fluorescence intensity