Figure 1.

Elevated BMI is associated with decreased expression of SIRT1 and increased acetyl–HIF-1α, HIF-1α, and aromatase in the breast. A, higher BMI correlates with decreased SIRT1 mRNA levels in breast tissue. B, obese women have decreased levels of SIRT1 mRNA in the breast compared with overweight or lean women (normal/underweight, n = 48; overweight, n = 33; obese, n = 19). Error bars, S.D. C, levels of SIRT1 and aromatase mRNAs are inversely correlated. RNA-Seq was used to quantify SIRT1 levels in A–C. D, immunoprecipitation followed by Western blotting demonstrated decreased levels of SIRT1 and increased levels of acetyl–HIF-1α, HIF-1α, and aromatase in breast tissue of obese (n = 6) versus lean (n = 6) women.