Figure 5.

Silencing PCAF inhibits PGE₂-mediated induction of aromatase. A–D, human breast ASC cell line was used. Cells were either untransfected or transfected as indicated with 2 μg of siRNA to GFP (control siRNA) or PCAF siRNA. A, 48 h after transfection, cells were harvested, and Western blotting was performed for PCAF, acetyl–HIF-1α, and β-actin. B–D, cells were treated with vehicle (control) or 500 nm PGE₂ for 24 h. Cell lysates were subjected to Western blotting, and the blots were probed as indicated.