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Bound Zn2+ and free thiol groups determined with TtRa2LD
All parameters were determined as described under “Experimental procedures.”
| Protein | [Protein] | [Zn2+] | Thiolfree/protein |
|---|---|---|---|
| nmol | nmol | ||
| TtRa2LD | 0.70 | 0.68 (0.97:1)a | 1.82 |
| Treated TtRa2LDb | 1.21 | 0.08 (0.07:1) | 5.90 |
a Ratio is between [Zn2+] and [protein].
b TtRa2LD protein was treated with EDTA/DTT.
