Table 2.
Dissociation constants (Kd) of wild-type and mutated TtRa2LD with two different DNAs
Values were determined with 2.5 nm DNA by steady-state FABA.
| Protein |
Kd |
|
|---|---|---|
| AH3–12 | AH3-ER6 | |
| nm | ||
| WT | 16.8 ± 1.0 | 10.7 ± 0.6 |
| E90A | 75.7 ± 4.3 | 26.7 ± 1.8 |
| L34A | 17.4 ± 1.2 | 12.3 ± 1.3 |
| R35A | 681 ± 118 | 407 ± 48 |
| R35H | 11.4 ± 1.0 | 12.9 ± 1.0 |
| R36A | 831 ± 111 | 623 ± 128 |
| R36H | 77.5 ± 6.7 | 43.3 ± 3.5 |
| K37A | 68.6 ± 5.2 | 28.3 ± 2.9 |
| K37H | 204 ± 19 | 151 ± 18 |
| TMa | NDb | ND |
| Δ123 | 27.8 ± 2.1 | 15.8 ± 1.2 |
| Δ113 | 90.8 ± 5.4 | 30.7 ± 2.1 |
| Δα5 | ND | ND |
a TM indicates triple mutant R35A/R36A/K37A.
b ND is not determined; values are too low to be determined unambiguously.