Figure 4.

Restriction of rEv37 on DENV-2 release from the endosome into the cytoplasm. A, effect of rEv37 on attachment was detected by confocal microscopy. Cells were pretreated with 10 μm rEv37 or 100 nm BAF for 24 h and then incubated with DENV-2 at 4 °C for 4 h for viral attachment. B, colocalization of DENV-2 particles and the late endosome. The pt-Dimer-Rab7 plasmid was transfected into Huh7 cells for 24 h, followed by treatment with 10 μm rEv37 for 24 h. The cells were transferred to 37 °C for 0.5 or 2 h after incubation with DENV-2 at 4 °C for 2 h. DENV-2 E protein/Rab7 colocalization was quantified and described as a percentage of total examined DENV-2 particles. Red, late endosomes; green, DENV-2 particles; and blue, cell nucleus. C, influence of rEv37 on H3 distribution. FITC-H3 cells were treated with 10 μm rEv37 or 100 nm BAF for 1 and 24 h, stained with DAPI, and observed under a confocal microscopy. H3 is a derived peptide of Ctry2459 characterized from C/ tryznai, and it enters the cell by low pH-dependent endocytosis. Scale bar, 10 μm.