Figure 2.

Substrate specificity and selectivity of S9Cdr. The enzyme assay was performed in 50 mm Tris-Cl, pH 8.0, at 37 °C with 2 mm substrate concentration. The specific activity for N- and/or C-blocked peptides (A), tripeptides (B), peptides with different chain length (C), and S9Cdr mutants toward Met-Leu-Ser (D) are shown. The error bars depict S.D. between the replicates (n = 3). Nondetectable activity is depicted by *.