Figure 2.
MBD3 is required for neural differentiation of ESCs. A, immunoblotting analysis for MBD3 and α-tubulin (as a loading control) in control shRNA-expressing cells and Mbd3 shRNA-expressing cells at day 0. B, effect of Mbd3 shRNAs on the number of ESCs. One day before viral infection (day −3), 2.5 × 105 cells were plated, and the cells were counted at day 0. C, qRT-PCR analysis. Each mRNA level was normalized to the β-actin level, and the value of control shRNA-expressing cells at day 0 was set to 1. D, cells at day 7 were stained for TUJ1 (left). The scale bars, 100 μm. The percentages of TUJ1-positive cells are shown (right). B–D, data are shown as the means ± S.D. (n = 3 independent experiments). *, p < 0.05 and **, p < 0.01. N.S., not significant. The p values were calculated using Student's unpaired two-tailed t tests compared with the control cells on the same day.