Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 6;294(1):257–268. doi: 10.1074/jbc.RA118.003500

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Lu et al.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

PMC Copyright notice

Figure 3. — The majority of polyQ aggregates do not colocalize with HDAC6 or microtubules. A, left panel, a section slice showing cytosolic aggregates (green) in HEK cells that are immunostained against HDAC6 (red). Middle panel, projected view from a 3-D rendering of the data corresponding to the marked region in the left panel. Right panel, magnified view of polyQ aggregate clusters contained in the region indicated in the middle panel to which HDAC6 is bound. Only a small fraction of the clusters colocalize with HDAC6. Scale bars from left to right, 4, 1, and 300 nm, respectively. B, left panel, a section slice of cytosolic aggregates (green) in HEK cells that are immunostained against α-tubulin (red). Middle panel, same field of view, projecting full 3-D data set. Right panel, magnified view of small polyQ clusters localized near microtubules (blue arrowheads). The yellow color indicates areas where particles are in close association. White arrows point to “free” aggregates not associated to microtubules (white arrowheads). Scale bars from left to right, 2, 2, and 400 nm, respectively.