Figure 3.

Effect of concentration and duration of DEX treatment on MP activation in mp MP-GR inflorescence stems. (A) Seedlings were germinated and grown on 0, 10, 30 or 60 μM DEX for 5, 10 or 15 d, and numbers of seed-bearing siliques on the primary inflorescence stems produced from each treatment were recorded. Columns represent mean values +/− SEM. Statistical significance of treatment comparisons was determined by Student t test analysis (*, P < 0.0005). The results show an increase in silique number with increasing duration of DEX treatment (see 5 d vs 10 d values) and increasing DEX concentration (see 15 d values). (B-E) Representative plants treated for 10 d on varying concentrations of DEX. The primary inflorescence stem of each plant is indicated (arrowhead), while seed-bearing siliques are designated with asterisks. In each case, at the depicted stage of development, the inflorescence stem has lost the ability to initiate functional flowers/siliques. Bars: 2 cm.