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. Author manuscript; available in PMC: 2019 Jan 7.
Published in final edited form as: Curr Biol. 2016 Feb 18;26(5):627–639. doi: 10.1016/j.cub.2016.01.011

Figure 2. Confirmation by Western Blot of the Presence in the MTL Complex of Proteins Identified by Mass Spectrometry in Photosynthetic Cell Cultures Grown Two Days in +Pi or −Pi Condition.

Figure 2.

20 μg of mitochondrial proteins were solubilized with 1.5 or 10 μg of β-DDM per mg of proteins and separated on CN-PAGE. The presence of a positive signal in the complex is indicated by an asterisk. A protein was considered as a component of the MTL complex if it was present in the MTL complex solubilized with 1.5 and 10 μg of DDM per μg of proteins. Some proteins (ATPase F1β, Miro1, and NDPK3) were detected by mass spectrometry because they migrate as a smear at the same level as the MTL complex on CN-PAGE, but they do not belong to it. The general function of the proteins analyzed and their mitochondrial localization are indicated. IM, inner membrane; IMS, intermembrane space; OM, outer membrane.