Figure 5.

Biotinylated peptide/MHC was incubated with PE-conjugated streptavidin to produce “small-scale” batches of SIINFEKL-reactive tetramers. Purified CD8+ T cells harvested from either wild-type or OT-1 mice were incubated with tetramers, washed, and stained with an anti-CD8 FITC antibody. Cells were again washed, fixed, and analyzed by flow cytometry for ligand binding. Abbreviations used: wild-type (WT), streptavidin (SA)