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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Immunol Methods. 2018 Oct 31;464:64–73. doi: 10.1016/j.jim.2018.10.013

Fig. 1.

Fig. 1.

In vitro sieving assay using artificial mixtures of molecular clones, and schema of the assay using deep sequence. (A) in vitro sieving assay using mixture of tier 1 (VTRS) and tier 2 (IAKN) SIVsmE660 IMC after infection in the presence of various concentrations of ITS01. Peaks in Sanger sequence at 45th and 47th residues in Env are shown. (B) The ratios of Tier 1:Tier 2 variants at the 4 positions in ENV are graphed for each condition. The proportions show an expected selection for (enrichment) neutralization-resistant, Tier 2 viruses, in the presence of higher amounts of ITS01. (C) Schema of in vitro sieving assay and deep sequence analysis.