Fig. 4.

Increased number of lysosomes in saposin A patient fibroblasts (proband) compared to healthy controls (father and brother). Immunofluorescence signals of LAMP1 were enhanced in fibroblast of proband (c) compared with her healthy family members (a, b, respectively). Lysosomes (green) were stained with Anti-Lamp1 antibody. Nuclei (blue) were stained with DAPI. 20× microscopic images. An increased number of LAMP1 vesicles in proband fibroblasts were analyzed using ImageJ (d). The data are represented as the mean ± S.E.M. One-way ANOVA was used for statistical analysis (**p < 0.025)