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. 2019 Jan 7;51(1):3. doi: 10.1038/s12276-018-0190-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative morphologies of HLA-B-engineered iPSC clones. Images were acquired on a Leica microscope (Leica Microsystems Ltd, EC3, image 40 × ). Expression analysis of stemness markers in the wild-type and HLA-B-engineered iPSC clones was performed at the b mRNA and c protein levels by RT-PCR and immunofluorescence staining, respectively (scale bar, 200 μm). d Teratoma assay with wild-type and HLA-B-engineered iPSCs (scale bar, 200 μm). e Genetic profiling of wild-type and HLA-B-engineered iPSC clones. STR was based on the multiplex analysis of 15 loci and the amelogenin gender-determination marker. f Representative karyotypes of wild-type and HLA-B-engineered iPSC clones