Fig. 4.

Increased serum NEFA and plasma triacylglycerol levels directly reduce serum Mg²⁺ concentrations in healthy overweight or obese women. Female volunteers underwent an oral fat (cream) loading test. (a) Serum Mg²⁺ (red circles) and plasma triacylglycerol (blue squares) and (b) serum Mg²⁺ (red circles) and NEFA (blue squares) concentrations before (0 h) and at 1, 2, 4, 6 and 8 h after an oral lipid load in healthy overweight women (n = 22). (c) Total serum Mg²⁺ levels measured by ICP-MS before (0 h) and 4 and 8 h after oral lipid intake. Data are mean ± SEM.*p ≤ 0.05 vs Mg²⁺ concentrations at 0 h; ^†p ≤ 0.05, ^††p ≤ 0.01, ^†††p ≤ 0.001 vs triacylglycerol or NEFA concentrations at 0 h. Significance was evaluated using a one-way ANOVA with Dunnett’s correction for multiple comparisons