Fig. 1. JNK-IN-8 promotes HSPC expansion in vitro.

a Experimental schematic for the evaluation of JNK inhibitors on HSC expansion. CD34⁺ cells were cultured with StemSpan SFEM II medium supplemented with SCF, TPO, FLT3L in the presence of JNK-related molecules for 7 days, then the total cell expansion and CD34⁺CD45RA^- cell frequency was determined. b Frequency of CD34⁺CD45RA^- cell subsets in 7-day cultures of CD34⁺ cells supplemented with two representative JNK inhibitors including JNK-IN-8 (2 μM) and SP600125 (5 μM) (n = 3 experiments). c Chemical structure for JNK-IN-8 (hereafter called J8). d The increasing fold of CD34⁺CD45RA^- subset frequency compared to DMSO after 10-day culture of CD34⁺ cells with different concentration of J8 (This data was drawn by R). See also to Supplementary Table S1. All data shown as mean values ± SD. Statistical significance was assessed using unpaired t test, where ***p < 0.001. See also Supplementary Fig. S1