Table 3.

Antibacterial activities of diclofenac-loaded chitosan nanoparticles (DIC.CNPs).

Microorganism	MIC90 (µg/mL)
	HMW.DIC.CNPs	LMW.DIC.CNPs	DIC
Staphylococcus aureus	18* ± 1.6	35* ± 2.2	250 ± 4
Bacillus subtilis	9* ± 1.2	17.5* ± 2.7	50 ± 3.1

^*P value <0.01, broth dilution method was used to determine the minimum inhibitory concentration (MIC₉₀) of prepared NPs against S. aureus and B. subtilis after 18 h incubation at 37 °C. Values are means of MIC₉₀ ± standard deviation (SD) of at least three experiments (n = 3).