CPT1C KO mice show an altered expression of markers of leptin signaling and ER stress in the mediobasal hypothalamus after short-term administration of a HF diet. (A–C) Protein levels of pSTAT3 (A), mRNA levels of SOCS3 (B) and protein expression of pAMPKα, pACC, AMPK, and ACC (C) in the mediobasal hypothalamus of WT and CPT1C KO mice fed a standard diet (SD) or a high fat (HF) diet for 7 days. (D) Protein levels of pAMPKα and AMPK in the mediobasal hypothalamus of WT and CPT1C KO mice after ICV administration of leptin or vehicle. (E) mRNA levels of ER stress markers in the mediobasal hypothalamus of WT and CPT1C KO mice fed a SD or a HF diet for 7 days. Data are expressed as mean ± SEM (n = 5–7). *P < 0.05, **P < 0.01 versus WT with the same diet; #P < 0.05 versus SD within the same genotype; +P < 0.01 versus vehicle within the same genotype.