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. 2018 Nov 2;19:75–85. doi: 10.1016/j.molmet.2018.10.010

Figure 5.

Figure 5

Expression of CPT1C in the VMH restores short-term diet-induced response in CPT1C KO mice. (A) GFP (empty vector, EV) or CPT1C-GFP (Cpt1c)-expressing lentiviruses were microinjected in the VMH of WT and CPT1C KO mice and after 1 week, mice were fed a standard diet (SD) or a high fat (HF) diet for 7 days. (B) Injection site was confirmed by direct fluorescence of GFP in brain sections or by CPT1C expression analysis by western blot in the ventral hypothalamus. (C–E) Body weight gain (C), plasma leptin (D) and gene expression analysis of thermogenic markers in BAT of WT-EV, KO-EV, and KO expressing CPT1C (KO-Cpt1c) or CPT1CM589S (KO-Mut) fed SD or HF diet for 7 days. Data are expressed as mean ± SEM (n = 6–8). *P < 0.05 versus WT-EV-HF; #P < 0.05 versus WT-EV-SD; +P < 0.05, +++P < 0.001 versus KO-EV-HF.