CPT1C KO mice show impaired AMPK-mediated effects within the VMH on body weight change, BAT thermogenesis and liver. (A) Body weight change of WT and CPT1C KO mice treated with adenoviruses encoding GFP (Empty vector, EV) or AMPK-DN in the VMH. (B–D) Representative infrared thermal images and quantification of interscapular temperature adjacent to the BAT depot (B), protein levels of UCP1 in BAT (C) and gene expression analysis of thermogenic markers in BAT of WT and KO mice treated with EV or AMPK-DN in the VMH (D). (E and F) Protein levels of the AMPK pathway (E) and TG levels in the liver of mice treated with EV or AMPK-DN in the VMH (G). Data are expressed as mean ± SEM (n = 6–7). *P < 0.05, **P < 0.01, ***P < 0.001 versus WT-EV; #P < 0.05, ##P < 0.01 versus WT-AMPK-DN.