Table II.
Gene | Primer sequence | PCR cycle condition |
---|---|---|
ACTL8 | Forward: 5′-GCCACGTGCTCACAGAGTAG-3′ | 95°C 5 sec |
Reverse: 5′-CTCAGCTGCACACTGCAAAC-3′ | 95°C 30 sec | |
GAPDH | Forward: 5′-GGAGCGAGATCCCTCCAAAAT-3′ | 60°C 45 sec |
Reverse: 5′-GGCTGTTGTCATACTTCTCATGG-3′ | 72°C 30 sec |
PCR, polymerase chain reaction; ACTL8, actin-like protein 8.