Figure 2.

Efficacy of Lv-shRPS15A in 786-O cells. (A) Green fluorescent protein-expressing cells were observed under a fluorescence microscope at 72 h post-transfection. (B) Expression levels of RPS15A mRNA in the Lv-shRPS15A- and Lv-shCtrl-transduced 786-O cells were analysed by reverse transcription-quantitative polymerase chain reaction. The results are represented as the mean ± standard deviation of three independent experiments; ***P<0.001. (C) Western blotting indicating a notable reduction of RPS15A protein expression in the transfected 786-O cells. Ctrl, control Lv, lentivirus; RPS15A, ribosomal protein S15A; sh, short hairpin RNA.