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Expression of a recombinant HCV E2 gene. Western blot detection of HCV E2 expression in 293 cells after transfection with pAAV.CMV.HCV E2 (lanes 1 and 2) or pAAV.CMV.eGFP (lanes 3 and 4). The HCV E2 protein is approximately 70 kDa. The protein GAPDH (36 kDa) was used as an internal control. The result shown is representative of three experiments.
