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Neutralizing antibody against AAV vector in human and mice sera. Serum samples from human and naive mice were diluted (1:20 and 1:80) and tested against AAV8 or AAVrh32.33. (A) The eGFP protein expression levels for each group indicated the NAb levels against AAV vectors (magnification, ×200). Samples were considered positive at a serum dilution of 1:20 (B) or 1:80 (C) when vector transduction was inhibited by 50%. *P<0.05; **P<0.01.
