Figure 1.

Effects of U0126, oxaliplatin and 5-FU on SW48 cells. Cell viability was measured using CCK-8 assay and is represented as the percentages of the untreated group value. (A) CCK-8 was performed following the treatment of SW48 and NCI-H508 cells with increasing concentrations of U0126 for 72 h. There was a statistical difference between the two groups (*P<0.05). (B) After 72 h of U0126 exposure, the cells were lysed and subjected to western blot analysis with relevant antibodies. Cell viability of cells treated with a concentration gradient of (C) oxaliplatin (0.5, 1, 5, 10, 20 and 50 µg/ml) and (D) 5-FU (0.5, 1, 5, 10, 20 and 50 µg/ml) for 3 days was assessed by CCK-8 assay. Values are expressed as the mean ± standard deviation of three individual measurements. *P<0.05 and **P<0.01, vs. the untreated control group. 5-FU, 5-fluorouracil; CCK-8, Cell Counting Kit-8; ERK, extracellular signal-regulated kinase; t-, total; p-, phosphorylated.