Figure 2.

U0126 increased oxaliplatin and 5-FU toxicity in SW48 cells. (A) Cytotoxic effect in SW48 cells treated with or without 2 µM U0126 in combination with different concentrations of (A) oxaliplatin or (B) 5-FU for 72 h, as determined by CCK-8 assay. (C) Flow cytometry results, representative of three separate experiments. (D) Apoptotic rates of SW48 cells following exposure to U0126 and/or oxaliplatin/5-FU are presented. The abbreviation U in the figure stands for U0126. Data are expressed as the mean ± standard deviation of three independent experiments, performed in triplicate. *P<0.05 and **P<0.01, vs. control group; ^##P<0.01 vs. treatment with oxaliplatin/5-FU alone. 5-FU, 5-fluorouracil; CCK-8, Cell Counting Kit-8.