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. 2018 Dec 24;2018:3753547. doi: 10.1155/2018/3753547

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Copyright © 2018 L. L. Alekseenko et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Resumed proliferation of quiescent and proliferating cells after HS. (a) Immunofluorescence assay of proliferating and quiescent eMSC with anti-Ki-67 antibodies. Nuclei were contrasted with DAPI; scale bar = 50 μm. (b) FACS analyses of cell cycle of proliferating and quiescent eMSC after HS. (c) FACS analyses of cell viability of proliferating and quiescent eMSC after HS. (d) RT-PCR analysis of the p21 level in proliferating and quiescent cells. Data of three independent experiments are presented. (e, f) X-Gal staining. The progeny of HS-survived cells from proliferating (e) and quiescent (f) cultures. Ob: 10x; scale bar = 140 μm.