Figure 5.
Alda-1 has the efficacy of ALDH2 activity activation and toxic aldehyde clearance during liver IR. (a) ALDH2 activity was observed in liver tissues at 6 and 12 hours after reperfusion or sham operation (n = 4–6). (b) Western blot analysis of 4HNE protein adduct expression in liver tissues at 6 and 12 hours after reperfusion or sham operation (β-actin is used as a loading control). (c, d) Representative sections of 4HNE staining and the numbers of 4HNE-positive cells in liver sections at 6 h after reperfusion or sham. PT: portal triads; CV: central veins. Scale bars: 50 μm. (e) The malondialdehyde (MDA) content was measured in liver tissues at 6 and 12 hours after reperfusion or sham operation. All data are shown as mean ± SEM (n = 4–6). ∗∗ P < 0.01, ∗ P < 0.05.