Figure 4.

Carboxyterminal Methylation of Mutant PP2A Cα Subunits

(A) HA-tagged WT and mutant Cα proteins were isolated from transfected HEK293T cells by HA pull-down. PP2A C subunit methylation was subsequently assessed by immunoblotting with a demethyl-specific anti-C monoclonal antibody. Immunoblot data from a single representative experiment are shown (n ≥ 4).

(B) The average ratios of the quantified demethyl-specific signals over the quantified anti-HA signals ± SEM are displayed (n ≥ 4) in relation to those of WT Cα (set at 100%). A one-sample t test (compare to 100%) was used for assessing statistical significance (^∗p ≤ 0.05, ^∗∗p ≤ 0.01).