qPCR analysis of Firmicutes/Bacteroidetes, Clostridium XIVa, and Clostridium IV (A) and bacterially produced butyryl-CoA:acetate CoA-transferase (but) (B) from cecal bacteria with vehicle or three doses BBR treatment. (C) Acetate, propionate, butyrate, and glucose levels in the cecal bacteria with vehicle or three doses of BBR treatment by 1H NMR analysis. Values are means ± S.D. (n = 5 per group, flow cytometry; n = 6 per group, qPCR and NMR). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.